This service uses PNGase-F treatment to confirm the presence of glycosylated residues on a protein. The diagnostic asparagine to aspartic acid signature resulting from deglycosylation by PNGase-F can be identified by mass spectrometry. This service is ideal for scientists working in the glycobiology area and can be a precursor to a more involved glyco proteomics studies offered through our Custom-works service.
The Glyco-screen approach was used to identify 9 glycosylation sites on invertase, an enzyme that catalyzes the hydrolysis of sucrose into glucose and fructose.
b) Peptides identified with PNGase-F + trypsin. Note the converted amino acids indicating sites of PNGase-F activity and therefore N-glycosylation.
MSB-19 can be ordered directly from our online ordering system, here.