New Promega/MS Bioworks Pubhub article

Identifying protein subunits is a routine application of mass spectrometry. With advances in this technology, characterization of complexes has become accessible to more researchers. Using the human proteasome as our model complex, we demonstrate how new commercial technologies and services allow researchers to quickly characterize a complex with little upfront effort. We combined HaloTag® technology with the services from the Kazusa Institute and MS Bioworks to rapidly identify and characterize subunits in the human proteasome. Using a luciferase-based assay, we were able to assay our complexes for protease activity before identifying the proteins using mass spectrometry. Finally, we used cell-free protein expression to quickly map protein interactions in the human proteasome. This integrated project design required no traditional cloning, little optimization and minimal hands-on time.

http://www.promega.com/resources/articles/pubhub/functional-proteomics-techniques-to-isolate-and-characterize-the-human-proteasome/

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