Targeted Proteomics

Overview

Our targeted protein quantitative services are ideal for studies focusing on changes in the level of a protein or a protein modification. Our Parallel Reaction Monitoring (PRM) offers a highly specific method for quantifying proteins and their post-translational modification with reduced assay development time and costs.

Steps Involved

These projects are typically target specific but a guide is provided below.

  • Sample preparation: Protein extraction and solution digestion with trypsin.
  • LC-MS/MS: 1hr LC-PRM/MS
  • Data analysis: Skyline
  • Report generation: PDF/Excel/Skyline

The turnaround time is 2-3 weeks.

Features and Deliverables

  • Written Report containing details of your samples, our methods and a summary of the data.
  • An Excel workbook containing the detailed qualitative and quantitative protein identification data.
  • A Skyline File to enable your own easy visualization of the data.

An example report is available upon request.