Proteins are digested with trypsin and the resulting peptides are analyzed by LC/MS/MS. The peptides are fragmented in the mass spectrometer to yield diagnostic patterns that can be matched to protein sequence databases via computer algorithms. The sensitivity of this technique is 1ng or less starting material for a given protein.
Solutions containing a simple buffer background (e.g. water or volatile salts) are amenable to direct in-solution digest; more complex backgrounds (e.g. detergent, non-volatile salts, glycerol) require an additional clean-up step to facilitate the sample analysis (MSB-02).
Sample types: 2D gel spots, SDS-PAGE bands, recombinant proteins, purified proteins
Data turn around: Two weeks or less
Report format: PDF report