Liquid Chromatography-Parallel Reaction Monitoring/Mass Spectrometry (LC-PRM/MS) is a technique for the targeted quantitation of proteins. The principle underlying this approach is one of surrogacy; a proteolytic peptide produced from the enzymatic digestion of a protein can be monitored and quantified using LC-PRM/MS. The calculated molar amount of the surrogate peptide can be used to infer the concentration of the protein, assuming a 1:1 mole ratio between the peptide and the protein.
This service is designed to enable access to this emerging technology for use on your own biological systems. This service involves the quantitation of ten proteins in a ten samples using single point calibration. Internal standard peptides are not included in the service but MS Bioworks is happy to assist in sourcing and purchasing suitable reagents.
The steps involved in a Parallel Reaction Monitoring experiment are:
Key features of a Parallel Reaction Monitoring experiment are: