Glyco-screen

Overview

This screening service can be used to determine presence or absence of N-glycosylation sites on an antibody, recombinant or highly purified protein. Modified asparagine (Asn) residues are converted to the deamidated aspartic acid (Asp) following treatment with PNGase F, this is a simple modification to pick up in a database search. Analysis without treatment accounts for any artifactual deamidation.

Steps Involved

• Sample preparation
• LC-MS/MS
• Data analysis
• Report generation: PDF/Excel

The turnaround time is 2-3 weeks.

Deliverables

• Written Report containing details of your samples, our methods and a summary of the data.
• An Excel workbook containing the detailed qualitative and quantitative protein identification data.
• A Scaffold file to enable your own deeper data mining.

An example report is available upon request.

A service note with some data outlining some fundamentals of the service is available here.