Phospho-screen

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Overview

This service enables a global view of protein and site specific phosphorylation events using a workflow optimized for a single shot 2hr-LC-MS/MS analysis. With lower costs, higher throughput and high sensitivity this workflow is rapidly emerging as an industry favorite for the study of systems wide modulation of phosphorylation.

Steps Involved

  • Sample preparation: Protein extraction, solution digestion with trypsin, enrichment of phosphopeptides with titanium dioxide.
  • LC-MS/MS: 2hr LC-MS/MS using a Q Exactive HF or Fusion Lumos.
  • Data analysis. Phosphorylation site identification quantitation with MaxQuant. Differential analysis with Perseus.
  • Report generation: PDF/Excel

The turnaround time is 2-3 weeks.

Deliverables

  • Written Report containing details of your samples, our methods and a summary of the data.
  • An Excel workbook containing the detailed qualitative and quantitative protein identification data.

An example report is available upon request.