PTM Profiling


This service enables the identification, localization and quantitation of post-translational modifications on a protein using a panel of three enzymes to maximize sequence coverage of the target protein.

Steps Involved

  • Sample preparation: Trypsin digestion of the submitted gel band.
  • LC-MS/MS: 0.5hr LC-MS/MS
  • Data analysis: Protein and PTM identification is performed with Mascot. Protein visualization and validation is performed with Scaffold. PTM Validation is performed with Scaffold PTM.
  • Percentage occupancy can be approximated using Byologic (ProteinMetrics)
  • Report generation: PDF/Excel/Scaffold + Scaffold PTM

The turnaround time is 2-3 weeks.


  • Written Report containing details of your samples, our methods and a summary of the data.
  • An Excel workbook containing the detailed qualitative and quantitative protein identification data.
  • Scaffold and Scaffold PTM files to enable your own deeper data mining.

An example report is available upon request.

A service note with some data outlining some fundamentals of the service is available here.