PTM Profiling

Overview

This service enables the identification, localization and quantitation of post-translational modifications on a protein using a panel of three enzymes to maximize sequence coverage of the target protein.

Steps Involved

• Sample preparation: Trypsin digestion of the submitted gel band.
• LC-MS/MS: 0.5hr LC-MS/MS
• Data analysis: Protein and PTM identification is performed with Mascot. Protein visualization and validation is performed with Scaffold. PTM Validation is performed with Scaffold PTM.
• Percentage occupancy can be approximated using Byologic (ProteinMetrics)
• Report generation: PDF/Excel/Scaffold + Scaffold PTM

The turnaround time is 2-3 weeks.

Deliverables

• Written Report containing details of your samples, our methods and a summary of the data.
• An Excel workbook containing the detailed qualitative and quantitative protein identification data.
• Scaffold and Scaffold PTM files to enable your own deeper data mining.

An example report is available upon request.

A service note with some data outlining some fundamentals of the service is available here.