Revolutionizing Spatial Proteomics
Introducing the World’s First High-Resolution Spatial Proteomics Service
MS Bioworks, OMAPiX, and Syncell are working together to bring you an industry-first spatial proteomics service—a groundbreaking innovation in biological discovery.
Recognized by Nature Methods as the 2024 Method of the Year, spatial proteomics is transforming our understanding of tissue organization and biological complexity.
Volume 21 | December 2024 | 2195–2196
Method of the Year 2024: spatial proteomics | Nature Methods
Cutting-Edge Technology, Unprecedented Insights
This powerful new service integrates:
Microscoop® Technology
Unbiased spatial proteomic discovery from Syncell
Spatial Omics Expertise
Proven methodologies from OMAPiX
Orbitrap Astral Proteomics
Unmatched sensitivity & precision from MS Bioworks
Together, we are redefining spatial proteomics—giving you unprecedented access to high-resolution protein constituent discovery and mapping at a level never before possible.
Discover the Future of Spatial Proteomics Today!
Our four-step workflow is described below:
Region of Interest (ROI) Selection and Image Analysis
Cells (Fixed, Frozen) or Tissue (FFPE, Fresh Frozen, PFA/Methanol fixed) are stained with a marker that the researcher uses to predefine areas for deeper proteomic analysis. This can be stained with an immunofluorescent marker, multiple markers or other anatomical marker of interest that can be identified via microscopy.
One can define these regions based on size, shape, location, distance, and intensity. The user places the stained sample on the Microscoop stage and the sample is imaged. These ROI’s are identified manually within one field of view (FOV) and converted by the Microscoop Autoscoop Software to an image mask, a binary parameter, that tells the system which specific locations to target for whole proteome analysis.
The image mask is automatically applied to the entire sample. The ROI’s are the “blueprint” by which the 2-photon laser uses in the next step to target the specific locations of interest.
Patterned Photo-Biotinylation
The Synlight Rich kit, containing a photobiotinylation reagent, is applied to the entire sample. A femtosecond light source is controlled mechatronically is used to illuminate the region of interest one pixel at a time.
This patterned illumination triggers targeted protein photo-biotinlyation with high spatial precision through the reactions of light-sensitive probes of the Synlight-Rich™ Kit. This patterned photolabeling is repeated for hundreds to thousands of FOVs automatically.
The number of photolabeling events is dictated by the size and quantity of the ROI’s. The intention is to aggregate all ROI protein material together to ensure there is sufficient protein to go through the protein extraction and input to downstream mass spectrometry or alternative downstream proteomic readouts.
This entire step is done automatically and requires no user intervention until all pixels are labeled by the laser across the FOV’s of interest.
Protein Extraction
The entire sample contents are collected from the slide or chamber. Materials from multiple slides or chambers can be pooled together to increase the total protein content. The Synpull Kit is used to carry out the next steps of extraction. Using the kit, the samples are lysed, biotinylated proteins are enriched and purified by immunoprecipitation, and proteins digested into peptides for proteomic analysis.
Proteomic Identification and Analysis
The collected peptides can be lyophilized for downstream proteomic analysis. Typically, an unbiased proteomic analysis of the protein lysate collected from the Synpull Assay is performed on a mass spectrometer (LC-MS/MS). Proteomes of both the photo-labeled and unlabeled (control) samples are obtained.
By comparing the proteomic makeup of the control and photolabeled samples, a location-specific proteome is obtained with high sensitivity and, high specificity with high spatial precision. Validation can be done by colocalization of immunostaining, subsequent follow-on mass spec experiments or additional functional assays.?
Unique analyses like differential protein expression, treatment/control, disease/normal, and post translational modifications can also be carried out with the output of the Microscoop.
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